Western Blot Detection System

2 0 system both accelerates immunodetection shortening time to results and enables parallel processing of blots making it easy to apply consistent conditions across experiments.

Western blot detection system.

Learn more about western blotting techniques. Western blot is a well established and widely used technique for the detection and analysis of proteins. The method is based on the construction of an antibody protein complex with specific antibodies that bind to proteins immobilized on a membrane. Western blotting is a tried and true technique in the life science lab for separating proteins based on their molecular weight.

The western blot is extensively used in biochemistry for the qualitative detection of single proteins and protein modifications such as post translational modifications at least 8 9 of all protein related publications are estimated to apply western blots. It is used as a general method to identify the presence of a specific single protein within a complex mixture of proteins. We offer comprehensive protein detection solutions designed for your western blotting success. Pierce ecl western blotting substrate provides reliability and performance equivalent to other standard ecl substrates for detection of hrp enzyme activity.

Find step by step protocols and helpful tips on equipment membranes transfer conditions and detection methods. The immunodetection phase of western blotting consisting of blocking antibody incubations and washing steps may be subject to experimental variability. The reaction uses a detection reagent containing lgbit protein which complements the hibit tag to form the luminescent nanobit enzyme. Quick visualization precise quantitation single probe detection or multiplexing we offer a range of reagents kits and instruments for western blot detection and analysis.

Protein separation and analysis. It enables the detection of picogram amounts of antigen and allows for easy detection of hrp using photographic or other imaging methods.